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dc.contributor.advisorPrice, David
dc.contributor.advisorMason, John
dc.contributor.advisorPratt, Thomas
dc.contributor.authorNowakowski, Tomasz Jan
dc.date.accessioned2014-10-03T12:56:13Z
dc.date.available2014-10-03T12:56:13Z
dc.date.issued2012-06-22
dc.identifier.urihttp://hdl.handle.net/1842/9471
dc.description.abstractDicer endoribonuclease catalyzes the maturation of microRNAs (miRNAs) from double stranded precursors. Studies conditionally inactivating Dicer in the mouse embryonic forebrain continue to shed light on the spectrum of biological processes subject to miRNA regulation. This study looked at defects of brain development following a widespread ablation of Dicer in the early forebrain. The neuroepithelial stem cells failed to specify the radial glia appropriately around the time when the first postmitotic neurons begin to be generated in the neuroepithelium. Ablation of Dicer in only a subset of radial glia was not accompanied by the early apoptosis observed in all other models of Dicer ablation in the cortex. This allowed the study of the role of miRNAs in regulating cell numbers in the cortex. The study revealed that generation of cortical cells is increased during postnatal development. Finally, the study identified a miRNA which is able to negatively regulate the development of neuronal precursor cells of the developing cortex by targeting Tbox transcription factor 2. Together the results presented in this Thesis contribute to the understanding of the roles of endogenous RNA interference in the development of the brain.en_US
dc.language.isoenen_US
dc.publisherThe University of Edinburghen_US
dc.relation.hasversionNowakowski, T. J., Mysiak, K. S., Pratt, T. and Price, D. J. (2011). Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon. PLoS One 6, e23013.en_US
dc.subjectmicroRNAen_US
dc.subjectdicer endoribonucleaseen_US
dc.subjectmiRNA regulationen_US
dc.subjectbrain developmenten_US
dc.titleRoles of the microRNA pathway in cortical developmenten_US
dc.typeThesis or Dissertationen_US
dc.type.qualificationlevelDoctoralen_US
dc.type.qualificationnamePhD Doctor of Philosophyen_US


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