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dc.contributor.authorColquhoun, Daviden
dc.date.accessioned2019-02-15T14:36:15Z
dc.date.available2019-02-15T14:36:15Z
dc.date.issued1964en
dc.identifier.urihttp://hdl.handle.net/1842/35252
dc.description.abstracten
dc.description.abstractA method of measuring adsorption without washing the tissue has been devised and studied.en
dc.description.abstractDiffusion through chopped lung tissue has been studied from a theoretical and experimental point of view. Predicted curves for the y-globulin content of the extracellular space have been calculated and applied to experimental results. The rate of uptake was found to be at least partly diffusion controlled. Adsorption equilibrium and rate constants have been rigorously defined. No evidence was found for a fast initial phase of sensitization or adsorption.en
dc.description.abstractCalculations have been performed concerning the consequences of slow steady y-globulin uptake after long periods of incubation.en
dc.description.abstractThe amounts of rabbit y-globulin adsorbed onto lung tissue were found to be of the same order of magnitude, or somewhat lower, than those previously reported.en
dc.description.abstractNo evidence against a linear adsorption isotherm was found in any experiments.en
dc.description.abstractNeither adsorption nor sensitization were altered by reducing the calcium concentration during passive sensitization.en
dc.description.abstractReduction of the ionic strength of the medium caused a large increase in the amount of Y-globulin adsorbed but did not increase sensitization.en
dc.description.abstractTwo guinea pig antibodies have been separated by preparative electrophoresis and ion exchange chromatography.en
dc.description.abstractThe antigenic relationship and purity of γ₁-and γ₂-globulins have been studied. No contamination was detectable in γ₂_globulin, but y₁-globulin contained fast γ₂-globulin and sometimes ß-globulins as well.en
dc.description.abstractOvalbumin (crystallized 5 times) has been shown to contain at least four proteins.en
dc.description.abstractIt has been shown that γ₁-globalin anti-bodies sensitize lung tissues in very low concentrations, but no evidence was found that the very low sensitizing power of γ₂-globulin antibodies was not due to impurity.en
dc.description.abstractQuantitative passive cutaneous anaphylaxis experiments performed in parallel with the tests on lung tissue showed that whenever the γ₂-globulin fraction contained antibody it appeared more potent relative to γ₂-globulin than when tested on lung tissue although the γ₁-globulin was always considerably more potent in both tests.en
dc.description.abstractThe skin sensitization produced by the γ₂--globulin fraction disappeared faster than that produced by the γ₁-globulin fraction.en
dc.description.abstractIt was not possible to detect enough γ₁-globulin contaminant in the γ₂-globulin fraction to account for the skin sensitizing ability of the latter.en
dc.description.abstractIt was concluded that γ₂-globulin antibody must have some skin sensitizing ability of its own, but that it is considerably less potent than the γ₁-globulin antibody.en
dc.description.abstractNo difference was detectable between the extents of adsorption of γ₁-and γ₂-globulins onto lung tissue.en
dc.description.abstractAn equation has been derived describing the loss of thiosulphate by radiative oxidation in iodine-131 solutions.en
dc.description.abstractAn analysis has been presented of interpolation and other errors in a rapid method for assaying large numbers of histamine solutions using visual linear interpolation between two standards.en
dc.publisherThe University of Edinburghen
dc.relation.isreferencedbyen
dc.subjectAnnexe Thesis Digitisation Project 2019 Block 22en
dc.titleThe characterization and adsorption of sensitizing antibodiesen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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