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dc.contributor.authorThomas, Jacoben
dc.date.accessioned2018-05-22T12:48:57Z
dc.date.available2018-05-22T12:48:57Z
dc.date.issued1977en
dc.identifier.urihttp://hdl.handle.net/1842/30829
dc.description.abstracten
dc.description.abstractA survey of the early literature on aberrant bacteria has shown that what are now considered to be L forms or cell wall defective variants were well recognised entities long before Klieneberger drew attention to the presence of these microbial variants in the cultures of Strcptobacillus moniliformis. The review of the more recent interdisciplinary investigations carried out on L forms and other wall defective microbial variants has revealed that there is still a considerable lack of information on their biological and pathogenic properties.en
dc.description.abstractInvestigations carried out on ten strains of Salm. gallinarum have shown penicillin, glycine and a number of other substances used in the present work are capable of inducing L transformation in this bacterial species. Considerable variations occurred between the strains in their ability to respond to L transformation. Isolaxes from more recent clinical material were found to undergo L transformation more readily than the standard laboratory strains. Likewise it was easier to induce L transformation in the smooth than the rough strains of Salm. gallinarum. The ability of field isolates to undergo spontaneous L transformation without the aid of any known incitant is believed to be a new finding that has not been recorded in the Salmonella group of organisms.en
dc.description.abstractA number of cultural and environmental factors that aid in the transformation and propagation of these L forms were investigated and discussed. All ten strains of Salm. gallinaruia produced only an unstable L growth on a solid hypertonic medium. A hitherto unrecorded finding is that in the two laboratory strains which, though unable to produce stable L growth, were able to do so in a liquid medium; furthermore the elimination of the serum requirement for stabilisation in a liquid medium is hoped will provide the basis for the future immunological and biochemical characterisation of the L forms of Salm. gallinarum.en
dc.description.abstractBiochemical investigations on some of the L forms and revertants arising from these altered variants showed that they resembled in general their parental forms denoting absence of mutational changes.en
dc.description.abstractFiltration studies have revealed that not all elements in L cultures are filterable.en
dc.description.abstractInvestigations on the ultrastructure of L forms of Salm. gallinarum show that the inducing agents used in the current studies bring about varying degrees of cell wall damage resulting in the production of cell wall defective variants not only varying in their sizes but also in the amount of cell wall layers retained.en
dc.description.abstractThe pathogenic properties exhibited by the ions table L forms of Salm. gallinarum were shown to be due to their reversion to the vegetative forms in vivo and the death of the experimental host was ascribed to the endotoxic properties of the organism.en
dc.publisherThe University of Edinburghen
dc.relation.isreferencedbyAlready catalogueden
dc.subjectAnnexe Thesis Digitisation Project 2018 Block 19en
dc.titleA study of bacterial L formsen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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