Following an introduction and review of literature
pertaining to relevant aspects of major histocompatibility systems
and a review of literature on the bovine system specifically, an
introduction to East Coast fever (ECF) and review of literature on
ECF immunology and lymphoblastoid cell line (LCL) vaccination is
The development and application of a technique for
selection of bovine lymphocyte antigen (BoLA) ~ defined
subpopulations within established LCLs transformed by the causative
organism of ECF (Theileria parva) is then described. The results are
discussed in the context of T.parva-preferred target cells in in
vitro transformation systems, and the possible application of the
technique in producing LCLs lacking BoLA expression is considered.
No evidence of modulation of BoLA phenotype (workshop specificities)
as a consequence of Theileria~induced transformation was found.
Two large scale LCL challenge experiments in cattle are
reported, in which degrees of compatibility between LCLs and
recipient cattle were quantified and the effect of this on the
responses to LCL inoculation and subsequent stabilate challenge,
assessed. Evidence is presented for the BoLA system being an
important factor in determining the response to LCL inoculation and
the generation of immunity to a second challenge with the homologous
parasite, when cattle are inoculated with low numbers of cells
Also presented are the results of a series of experiments
carried out to investigate the specificity of bovine alloreactive
cytotoxic cells generated in vitro. The results suggest that BoLA
workshop specificities, as defined by alloantisera, are restrictive
in this system. The results also suggest that effects due to
expression of the products of a BoLA locus other than that coding for
the workshop specificities were being detected in some experiments.
The significance of these results for future studies of the
bovine immune system in general, and for studies of the immune
response in ECF in particular, is discussed.