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dc.contributor.authorYoung, Joannaen
dc.date.accessioned2018-03-29T12:21:07Z
dc.date.available2018-03-29T12:21:07Z
dc.date.issued2009en
dc.identifier.urihttp://hdl.handle.net/1842/29435
dc.description.abstracten
dc.description.abstractThe distinct structure of the Central Complex (CC) spanning the midline is one of the most prominent features of the Drosophila brain. The CC is highly conserved across insect species and is thought to be required for several behaviours. Although analyses have been performed on the organisation of this structure in the adult brain, few investigations have addressed its ontogeny. Unlike the Mushroom bodies, the CC is an exclusively imaginal structure differentiating in the early half of metamorphosis. Characterisation of the development and neuroarchitecture of this structure is critical for elucidation of function. By employing a combination of immunohistochemical techniques, enhancer trap technology and mutant analysis, this thesis has analysed the cellular structure and development of the CC.en
dc.description.abstractIn this study the expression patterns from a subset of genes encoding cell adhesion molecules generated three developmental series datasets from which a developmental timeline for the CC was determined. The echinoid gene displayed elevated expression in the Horizontal fibre system allowing a detailed developmental characterisation of this set of isomorphic neurons. Analysis using enhancer trap lines revealed genetic subdivision of neurons and isolation of several isomorphic CC neuron sets in the adult brain. These lines were subsequently used to assess the development of CC neurons. These experiments determined CC development to be in the first 48 hours after puparium formation. The CC neuron structure is established in a series of increments, with sets of isomorphic neurons projecting to their targets within defined time intervals commencing with the Horizontal and Vertical Fibre Systems.en
dc.description.abstractThis study includes details of CC structure indicated by projection patterns of neurons and by mutant analysis. In addition, the investigation of one of the genes involved in development, echinoid, is further analysed for a potential pathfinding function in the Optic lobe. Finally, this thesis presents the advantages conferred by the use of enhancer trap lines for developmental study of the CC and has isolated a novel set of these lines that will be beneficial for future studies into structure, development and behaviour of the CC.en
dc.publisherThe University of Edinburghen
dc.relation.isreferencedbyAlready catalogueden
dc.subjectAnnexe Thesis Digitisation Project 2018 Block 17en
dc.titleGenetic and developmental analysis of the Drosophila Central Complex: generation and organisation of distinct neuronal subsetsen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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