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dc.contributor.authorEvans, Anthony Marken
dc.date.accessioned2018-01-31T11:43:17Z
dc.date.available2018-01-31T11:43:17Z
dc.date.issued1990en
dc.identifier.urihttp://hdl.handle.net/1842/27986
dc.description.abstractA study has been made of the secretory response and the electrical response (a hyperpolarization followed by a depolarization) mediated by dopamine receptors of the cockroach (Nauphoeta cinerea Olivier) salivary gland in- vitro.en
dc.description.abstractReceptor classificationen
dc.description.abstractThe selective D1 agonists fenoldopam and SK38393, and the selective D2 agonists LY163502 and quinpirole induced, in descending order of potency, secretion and a concomitant electrical response from the acinar cells. The secretory and electrical responses to dopamine were found to be antagonised by, in descending order of potency, chlorpromazine (a non - selective dopamine antagonist), SCH23390 (a selective D1 antagonist), haloperidol and metaclopramide (selective D2 antagonists). Another D2 antagonist (p)sulpiride and the highly selective D2 antagonist domperidone did not inhibit either the secretory or electrical responses. These results taken together suggest that the receptor mediating the secretory and electrical response are the same, and analogous to the mammalian D1 sub -type. This suggestion is strengthened by the fact that SCH23390 inhibited the secretory and electrical response to all the selective agonists, while (p)sulpiride did not.en
dc.description.abstractAlthough domperidone did response to dopamine, three other actions were observed: one, post- synaptic, led to the potentiation of the hyperpolarization; this action was shared by (p)sulpiride. A separate post- synaptic action resulted in the inhibition of the depolarizing phase of the response. Finally a presynaptic action led to the abolition of the response to nerve stimulation.en
dc.description.abstractEffects of the calmodulin antagonists W7 and calmidazoliumen
dc.description.abstractIn an attempt to investigate the role of calmodulin in stimulus - secretion coupling within the salivary gland, the actions of two calmodulin antagonists, W7 and calmidazolium, were studied. In high concentrations, but within the range in which they are known to inhibit calmodulin, W7 and calmidazolium were found to inhibit dopamine- induced secretion and hyperpolarize the acinar cells. The hyperpolarization was not inhibited by SCH23390, and resulted from an increase in cytosolic free calcium, released from the same source as that accessed by dopamine. Lower concentrations of these two antagonists caused submaximal secretion and potentiated dopamine - induced hyperpolarizations. An interpretation of these results is that calmodulin promotes secretion, and exerts a negative control on cytosolic free calcium by an independent process which can be selectively inhibited.en
dc.publisherThe University of Edinburghen
dc.relation.isreferencedbyAlready catalogueden
dc.subjectAnnexe Thesis Digitisation Project 2017 Block 16en
dc.titleThe dopamine receptors of the cockroach salivary glanden
dc.typeThesis or Dissertationen
dc.type.qualificationlevelen
dc.type.qualificationnamePhD Doctor of Philosophyen


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