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dc.contributor.authorDuthie, Robert Buchanen
dc.date.accessioned2018-01-31T11:43:05Z
dc.date.available2018-01-31T11:43:05Z
dc.date.issued1956en
dc.identifier.urihttp://hdl.handle.net/1842/27962
dc.description.abstracten
dc.description.abstractAlthough the histology of post -foetal osteogenesis is well recognised, much uncertainty still remains about the fundamental problems of the causal genesis, the differential potencies and activities, and the metabolism of the osteogenic cells in bone growth and repair.en
dc.description.abstractIn the past, the histochemistry of osteogenesis has been limited to the inorganic phase of mineralisation and there has been remarkably little work on the preceding phase of the stimulus to cellular differentiation, and on the organic phase which is concerned with the pre-osseous cellular metabolism and elaboration of the bone matrix and is the precursor stage for the inorganic phase of new bone formation. An important intercellular substance of this organic phase is the sulphated mucopolysaccharide complex, chondroitin sulphuric acid, which is secreted by the osteogenic cells - the osteoblast and the chondroblast.en
dc.description.abstractIn the present study the importance of this chemical substance is emphasised because it controls and indeed identifies the morphological appearance of the cartilage cell and hence their possible origin, and because is is closely related to the subsequent deposition of the calcium phosphate complexes by acting as a "calcium- phosphate acceptor" in the bone matrix.en
dc.description.abstractA histochemical method is described of identifying chondroitin sulphuric acid in undecalcified bone sections which were obtained by an original technique. The histochemical localisation of this substance was obtained by staining for metachromasia and by "labelling" the sulphate radicle in the complex with radioactive sulphur with the process of autoradiography. The two techniques were complementary to each other and provided a high degree of specificity of localisation of chondroitin sulphuric acid. The accuracy of this method is discussed.en
dc.description.abstractBy serial sacrifice of groups of rats in which a uniform fracture had been inflicted, the chondroitin sulphuric acid complex was studied histochemically in vivo during epiphyseal growth and repair of the fracture. Further analysis of the normal pattern of this substance was obtained by producing abnormal hormonal states by the administration o oestradiol dipropionate, parathormone and thyroxin; an abnormal nutritional state of hypervitaminosis A; altered vascularity by the use of a local haemostatic substance Adrenoxyl and by ligation; a mechanical effect of internal fixation with use of an intrame dullary nail; and an enzymatic action by the local application of testicular hyaluronidase. The varying effects are recorded, correlated and discussed, especially from the morphogenetic aspect.en
dc.description.abstractThe significance of the accumulation of mast cells in the vicinity of the fracture as the source of secretion of histamine to act as the stimulus for cellular differentiation was studied after the local injection of a histamine- liberat.: substance - Compound 48/80.en
dc.description.abstractThe problem of resorption of the organic phase of bone structure was studied histochemically after the injection of a known chelating agent - ethylene -diamine tetra - acetic acid - and the negative results are considered.en
dc.description.abstractDuring the last two hundred years a large volume of experimental work has been undertaken to solve the fundamental problems of osteogenesis by studying the transplantation of osseous tissue. However, such work has been of little value because of the lack of histochemical information about the osteogenic cells and also because of the inability to define and to discriminate accurately between the relative osteogenetic activities and properties of the tissue transferred and those of the host or the environment of the tissue transfer.en
dc.description.abstractIn the present study there are recorded for the first time histochemical results from tissue culture in vivo of various forms of osteogenic cells whose activity was clearly defined by a physical and cellular barrier from the tissue of the host. From Algire's original diffusion chamber technique, a new method of tissue culture in vivo of post - embryonic tissues, which consisted of a diffusion chamber made of Millipore filter membranes, was developed and is described in detail. The physico -chemical properties of these filter membranes were accurately evaluated.en
dc.description.abstractThe histochemistry of post-foetal osteogenesis with particular reference to the metabolise of chondroitin sulphuric acid was studied during the processes of endochondral and intramembranous ossification resulting from the survival and differentiation of osteogenic cells of a fracture callus and of periosteum by this tissue culture technique in vivo. The osteogenetic potency and activity of various osseous transfers including cortical bone were studied and the cellular contents of the haversian systems in cortical bone were shown by their metabolism of radioactive chondroitin sulphuric acid to survive for at least seven days after transplantation.en
dc.description.abstractTo demonstrate the process of induction, an attempt was made to discover the possible secretion and passage into the surrounding host tissues of a specific osteogenic inductor substance from cells which exhibited osteogenetic activity within a diffusion chamber. Since the process of induction was not observed to take place, the necessity for the presence of a physico - chemical pathway for the transmission of any specific inductor substance is emphasised and the general reaction of the relative properties of the host tissue to those of the tissue transfers is discussed.en
dc.description.abstractAlthough the results from the present study are not immediately applicable to clinical problems, their consideration will lead to further understanding of the host -tissue transfer relationship as well as of the histochemical nature and importance of the organic phase of post-foetal osteogenesis.en
dc.publisherThe University of Edinburghen
dc.relation.isreferencedbyAlready catalogueden
dc.subjectAnnexe Thesis Digitisation Project 2017 Block 16en
dc.titleA histochemical study of the organic phase of post-foetal osteogenesisen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelen
dc.type.qualificationnameChM Master of Surgeryen


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