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dc.contributor.advisorDutia, Bernadette
dc.contributor.advisorConnelley, Timothy
dc.contributor.authorWang, Yu
dc.date.accessioned2017-11-16T14:15:10Z
dc.date.available2017-11-16T14:15:10Z
dc.date.issued2017-07-08
dc.identifier.urihttp://hdl.handle.net/1842/25520
dc.description.abstractNKp46+ CD3+ T cells have been defined as a novel non-conventional T lymphocyte subset of cattle that express both NK cell and T cell receptors. It has been hypothesized that NKp46+ CD3+ cells may form a niche bridging the innate and adaptive immune response and there is now evidence they may play a role in the responses against Theileria parva and Mycobacteria. Thus, NKp46+ CD3+ cells may offer a novel population to target in vaccination strategies. In bovine, recent studies have shown that NKp46+ CD3+ cells can recognize and respond to autologous Theileria parva infected cells (TpM) and that NKp46+ CD3+ cell lines can be generated and maintained in vitro. Functional analysis has indicated that both NKp46 receptor and CD3 (i.e. TCR) cross-linking can lead to cell activation, however, the function of the T cell receptor is still not clear. The aim of this study is to analyze the TCR repertoire of TpM-stimulated NKp46+CD3+ populations to look for evidence of TCR selection that would indicate a role for TCR in mediating recognition of TpM. To address this aim, we use high resolution NGS TRB sequence analysis to compare the TCR repertoires observed in TpM-stimulated and non-specific stimulated NKp46+CD3+ populations derived from naïve and T. parva-immune animals. TpM specific-stimulated NKp46+CD3+ cell lines were successfully generated from naive and T. parva immunized cattle, but generation and maintenance of NKp46+CD3+ cell lines through use of non-specific stimulant was unsuccessful. The results of this study provide evidence of clonal selection in the T. parva-specific response of NKp46+ CD3+ T-cells and in 2 naïve animals highly overlapping TRB repertoires. From the current data it is confirmed that NKp46+CD3+ cells express a highly diverse TRB repertoire with no obvious and consistent overt bias for usage of particular TRBV or TRBJ genes.en
dc.language.isoenen
dc.publisherThe University of Edinburghen
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectimmunologyen
dc.subjectNKp46+ CD3+ T cellsen
dc.subjectT cell receptoren
dc.titleInvestigating the clonality and formation of memory populations of non-conventional NKp46+ CD3+ T-cellsen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelMastersen
dc.type.qualificationnameMSc(R) Master of Science by Researchen


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