|
|
Edinburgh Research Archive >
Biomedical Sciences, School of >
Biomedical Sciences publications >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/1842/758
|
| Title: | Cdk5 is essential for synaptic vesicle endocytosis |
| Authors: | Tan, Timothy C
Valova, Valentina A
Malladi, Chandra S
Graham, Mark E
Berven, Leise A
Jupp, Orla J
Hansra, Gurdip
McClure, Sonya J
Sarcevic, Boris
Boadle, Ross A
Larsen, Martin R
Cousin, Michael A
Robinson, Phillip J |
| Issue Date: | Aug-2003 |
| Citation: | Cdk5 is essential for synaptic vesicle endocytosis Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ NATURE CELL BIOLOGY 5 (8): 701-710 AUG 2003 |
| Publisher: | Nature Publishing Group |
| Abstract: | Synaptic vesicle endocytosis (SVE) is triggered by calcineurin-mediated dephosphorylation of the dephosphin proteins. SVE is
maintained by the subsequent rephosphorylation of the dephosphins by unidentified protein kinases. Here, we show that cyclindependent
kinase 5 (Cdk5) phosphorylates dynamin I on Ser 774 and Ser 778 in vitro, which are identical to its endogenous
phosphorylation sites in vivo. Cdk5 antagonists and expression of dominant-negative Cdk5 block phosphorylation of dynamin I,
but not of amphiphysin or AP180, in nerve terminals and inhibit SVE. Thus Cdk5 has an essential role in SVE and is the first
dephosphin kinase identified in nerve terminals. |
| Keywords: | Synaptic vesicle endocytosis
cyclindependent kinase 5 |
| URI: | doi:10.1038/ncb1020
http://www.nature.com/ncb/
http://hdl.handle.net/1842/758 |
| Appears in Collections: | Biomedical Sciences publications
|
Items in ERA are protected by copyright, with all rights reserved, unless otherwise indicated.
|
